Ataxia | - Causing Protein Interaction Network Supplemental Data Site

Description of the study

This study reports a protein interaction network comprised of direct and indirect interactions of human ataxia disease-causing proteins. The ataxia proteins investigated are known to be involved with Purkinje cell degeneration in the human cerebellum. The data were obtained through a collaboration between the Zoghbi Laboratory at Baylor College of Medicine in Houston, Texas and the Vidal laboratory at Dana-Farber Cancer Institute at Harvard University in Boston, Massachusetts. The experimental design consists of 3 arms: a detailed yeast 2 hybrid (Y2H) analysis using cDNA derived protein fragments of the ataxia-causing proteins as baits; a full length protein Y2H study using the ataxia-causing proteins and the reagents of the hORFeome v1.1 (Rual et al. 2005); and an extensive literature and cross species analysis to identify additional protein interactions of the ataxia-causing proteins and their experimentally derived interactors. The full network consists of 3607 proteins with 6853 non-homodimer interactions. There were 119 homo-dimer interactions.

Data available through this site

This site contains all the data obtained in the course of this study. The raw data on experimentally derived protein interactions are available as well the literature and interologous interactions. Gene Ontology (GO) based analysis to characterize the co-annotation status of each interaction is also available. In addition to these data tables, GO enrichment analysis of the content of the interaction network is also available. The content analysis considers both the network as a whole and each individual disease-causing protein. The site contains a supplemental network comprised of non-related proteins. This so-called unrelated disease network serves as a control for analysis of the topological properties of our result. Finally, the web-site provides access to an interactive software tool for browsing the ataxia-disease network.